根结线虫的研究现状

概述了根结线虫的发生分布和传统分类鉴定,以及分子生物学技术(同工酶电泳技术、DNA重组技术、PCR技术)在根结线虫种和生理小种鉴定中的运用及其防治,并对生物防治及抗根结线虫育种、转基因工程在植物线虫学研究领域上的应用前景作了展望。     

亚洲玉米螟大量饲养中微孢子虫病控制技术

利用与人工饲料相混合的方法，比较了不同剂量的烟曲霉素B对亚洲玉米螟微孢子虫病的控制效果。结果表明，烟曲霉素B453、565和683μg/g的剂量处理都显著降低了亚洲玉米螟微孢子虫病的感染率和感染强度。食药1代时，幼虫和成虫感染率分别比对照平均减少44．4％—61．1％和41．7％—50％，其感染强度都由原来的严重感染分别下降为轻度感染。幼虫存活率、化蛹率、成虫繁殖力也均显著高于对照。连续处理3代饲喂烟曲霉素B对玉米螟生长发育没有不利影响，对微孢子虫病的控制作用随处理世代数的增加亦有增加的趋势。不同剂量的烟曲霉素B处理间的防效差异不显著，453μg/g可作为亚洲玉米螟大量饲养中控制微孢子虫病的推荐剂量。     

南方灭鼠应用稻谷与大米毒饵的比较

生物测定法测得谷壳对复方88-1灭鼠剂的阻滞率为54．3％，光谱分析法测得其对敌鼠钠盐的阻滞率在47．43％—57．00％之间。相同条件下，褐家鼠Rattus norvegicus取食敌鼠钠盐稻谷毒饵量为大米毒饵的2倍多。在南方潮湿的环境下，大米毒饵更易松散、霉变，使其适口性更低。综合考虑认为南方灭鼠使用稻谷毒饵更为合适。     

O3对日光温室黄瓜病虫害的防治效果

通过室内与大田试验，明确O3对日光温室黄瓜病虫害的防治效果。研究结果表明，在病害发生初期，用O3发生器隔天施放O3，每天施放3次，每次40min，间隔20min，O3浓度维持约1mg／kg，施放后7天，对黄瓜霜霉病、白粉病、细菌性角斑病及灰霉病防治效果分别为63．4％、53．6％、68．7％和65．0％。室内施放O3 60s，间隔20min，持续12h，对温室白粉虱、南美斑潜蝇成虫杀伤力为100％。O3对黄瓜植株安全，但随着黄瓜生育期的推进，对O3的忍受能力依次减弱。苗期忍耐O3最大浓度为1～2mg／kg，结果期0．4～1mg/kg，后期0．2～0．4mg／kg。O3防治黄瓜病虫害新技术具有安全、有效、方便、无二次污染等特点，尤其在黄瓜定植前对棚室进行大剂量消毒，可不留死角，且有效地解决了硫磺等药物消毒所造成棚内一些设施的破坏及对生态环境污染的难题，具有广阔的推广应用前景。     

Control of the cowpea aphid, Aphis craccivora Koch (Homoptera: Aphididae), in cowpea, Vigna unguiculata (L.) Walp.

Cowpea, Vigna unguiculata (L.) Walpers, is a major dietary staple in tropical Africa where it is most often cultivated. The production is, however, greatly hampered by severe infestation and damage by insect pests including the cowpea aphid, Aphis craccivora Koch. The damage and bionomics of the aphid are briefly introduced. Chemical, cultural, physical and biological methods, and use of plant resistance for A. craccivora control in cowpea are reviewed. Finally, prospects for integrated pest management are discussed     

Herbicide options for weed control in papaya

This paper reviews the literature on weeds and weed control in papaya. There is limited research on this topic, and nearly all of the research focuses on herbicides. Effective use of paraquat and glyphosate in papaya is dependent on avoidance of spray contact to green bark and foliage. Pre-emergence herbicide tolerance is dependent on papaya age, size and maturity, and soil type. Only one herbicide, oryzalin is shown to be tolerated by papaya immediately after transplanting. Herbicides such as diuron and oxyfluorfen with a broader spectrum of weed control generally injure young papaya, however they can be effectively used if the initial application of these herbicides is delayed until papaya attains certain size or maturity indices. There is a need for further research on weeds and weed control to improve the efficiency of papaya production     

Leafhopper and planthopper vectors of rice viruses and their management in Nepal

Research on insect pests (including leafhoppers and planthoppers) in Nepal was initiated in 1956 by the Entomology Division and Plant Protection Units of the Department of Agriculture. More systematic work began after the establishment of a Rice Improvement Programme in 1972. Because of poor documentation and publication of research findings, it is difficult to assess the research efforts and achievements to date. The available research reports are mostly published in the proceedings of national seminars/workshops. They are used here to assess the current status of research on the occurrence and damage caused, distribution and composition, relative abundance, biology and management through chemicals. Much of past research has focused on insect surveys by light traps and sweep nets and the evaluation of insecticides and, since early 1990, some research on rice green leafhoppers (Nephotettix virescens and Nephotettix nigropictus) has begun to determine their relative abundance and population characteristics in relation to their potential as vectors of rice viruses. The challenges to pest management in general and some suggestions for future research needs are discussed, based on the present standing of agricultural research in the country     

三种外检植物病毒的灭活处理

番茄环斑病毒（ＴｍＲＳＶ），烟草环斑病毒（ＴＲＳＶ），南芥菜花叶病毒（ＡｒＭＶ）的病汁液和ＰＥＧ粗提纯液，经适宜温度或甲醛处理，均丧失侵染性而有抗原性。ＴｍＲＳＶＰＥＧ粗提纯液经６０℃（水浴）处理１０分钟或２８℃７天，ＴＲＳＶ粗提纯液置２５℃一个月，Ａｒ－ＭＶ在４０℃７天条件下均可做为阳性对照的灭活处理的最佳方案。可保存抗原性在７～１２月以上。为了防止危险性检疫病毒的侵入，对入境种苗进行检疫，需建立快速、准确、标准化检疫程序，而在血清学快速诊断试验中，提供阳性对照，对提高判断准确率是必要的。因此，为了解决在诊断试剂中提供阳性对照但又不能使其检疫性病毒人为扩散这一重要问题，我们在研究三种外检病毒（ＴｍＲＳＶ，ＴＲＳＶ，ＡｒＭＶ）的检验技术的同时，又进行了一些灭活试验，用化学和物理方法钝化病毒，使其失去侵染性而保持抗原性，并应用于酶联诊断试剂盒中，本文报道了初步试验结果     

春麦田除草剂的应用与杂草群落演替

作者采用定点定位试验,对春麦田除草剂应用与杂草群落演替趋势进行研究。结果表明:在杂草群落多样性麦田,小麦连作并分别连续5年施用同一种除草剂,原杂草群落中占优势的靶标杂草得以控制,而非靶标杂草和抗、耐药性杂草因失去竞争和制约对象而猖獗发展,发生量较原来增加几倍至几十倍,并形成优势种群,对小麦造成新的更严重的危害。一种除草剂在同田块连续施用4年,由于杂草群落演替,抗、耐药杂草兴起,除草效果显著下降而失去其使用意义。作者提出除草剂配套使用、轮用、混用等措施,并配合以合理的轮作制度,以减轻杂草群落长期受到单一的定向选择性压力。     

生物安全与家禽疫病控制

疫病成为制约我国畜牧业发展的主要因素，要较好控制疫病必须推行生物安全措施。本文从生物安全概念、推行生物安全必要性、生物安全范围、生物安全难点及养鸡场生物安全措施进行了论述。